Nikon AXR confocal microscope gives high resolution and fluorescence imaging with a 100 nm resolution with Nikon Spatial Array Confocal (NSPARC) detector. With a largest Field of view (25 mm) coupled with scanning sizes up to 8k x 8k, sampling beyond the optical diffraction limit is possible even at low magnifications.
Model: AXR Ti2E
Manufacturer: NIKON
Specifications of the equipment
Objectives 4x, 10x, 20x, 40x, 60x (oil immersion) and 100x (oil immersion)
Lasers for confocal 405 nm, 445 nm, 488 nm, 514 nm, 561 nm and 640 nm.
Lasers for Total internal reflection fluorescence (TIRF) 405 nm, 488 nm, 561 nm and 640 nm.
Galvano Scanning provides the option of image stitching with highest resolutions.
Nikon Spatial Array Confocal (NSPARC) detector with 100 nm lateral resolution and 300 nm axial resolution.
Continuously variable pinhole.
Z step is 0.01 μm, 0.02 μm (with encoder control).
Z stacking, Total internal reflection fluorescence (TIRF), Fluorescence lifetime imaging Microscopy (FLIM), Fluorescence resonance energy transfer (FRET), Fluorescence recovery after photobleaching (FRAP) and Differential interference contrast (DIC) and Montage.
Live Cell imaging with incubation stage (CO2 flow, temperature and humidity).
Location:
Room 004 (Ground Floor), RCC,
IIT Hyderabad